GSM5218280: Gar15-13 Vehicle 3 ER; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
ESR1
Cell type
Cell type Class
Breast
Cell type
Breast cancer cells
NA
NA
Attributes by original data submitter
Sample
source_name
Gar15-13 Vehicle tumour
tissue
Breast cancer PDX model Gar15-13
treatment
Vehicle
replicate
3
chip antibody
ER (Millipore SC-543X)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Samples snap-frozen in Optimal Cutting Temperature compound (Tissue-Tek) were used for ER and FOXA1 ChIP-seq experiments. Using a cryostat (Leica, #CM3050-S), 15x 30 µm sections were cut from each tumour at -20 ̊C and these samples were subjected to double fixation with DSG and formaldehyde as previously described (Singh et al., 2019). Library preparation for ChIP-Seq was performed using the QIAseq Ultralow Input Library Kit (Qiagen, #180495).